Fig. 6. POPX2 knock-down causes rearrangement of the actin cytoskeleton and loss of stress fibres. (A) HeLa cells were microinjected with POPX2 siRNA and immunostained with rhodamine-coupled phalloidin. (B) HeLa cells were transfected with combinations of pXJ constructs of GFP or KID and POPX2 siRNAs. The cells were immunostained as in A. Three transfections were performed per construct. More than 100 cells were scored for each transfection. Lane 1, GFP; 2, GFP + control siRNA; 3, GFP + POPX2; 4, GFP + POPX2-siRNA1; 5, GFP + POPX2-siRNA2; 6, KID + POPX2-siRNA1. Scale bars: 10 µm.