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Figure 3


Fig. 3. Sphingolipid levels are reduced in the TbSPT2 RNAi cell line and in the 29-13 T. brucei cell line treated with myriocin (Myr). Negative-ion mode ESI-MS spectra of total lipids obtained from procyclic T. brucei are shown. 29-13, procyclic 29-13 cell line; 29-13 + Myr, 29-13 cell line treated with 1.5 µM myriocin; TbSPT2 + Tet, SPT2-RNAi-induced transfectant cells; TbSPT2–Tet, SPT2-RNAi-uninduced transfectant cells. m/z, mass to charge ratio; IPCs, inositolphosphorylceramides; PIs, phosphatidylinositols.