Fig. 5. Ultrastructural abnormalities of the TbSPT2 RNAi cell line. (A-H) Transmission electron microscopy images of (A) T. brucei 29-13 cells and of (B-H) cells of the TbSPT2 RNAi cell line grown for either 3 days (B,C) or 6 days (D-H) in the presence of Tet. Scale bars, 1 µm. (A) T. brucei 29-13 cell showing an undivided kinetoplast (K) next to the flagellar pocket (FP), a normal nucleus (N) and acidocalcisomes (A). (B) Cell with an abnormally large misshapen kinetoplast. (C) Cell with multiple nuclei. (D) Two cytoplasmic lipid deposits (^*), one surrounded by the endoplasmic reticulum and one surrounded by a coiled mitochondrion (M). Mitochondrial distention is observed at the top and bottom aspects of the cell. Acidocalcisomes (center left) appear normal. (E) Multilamellar structure (arrowhead) contiguous with the kinetoplast (K) membrane. (F) Fragmented Golgi complex next to the ER, and several cross-sections of a distended mitochondrion. (G) Coiled multilamellar structure (arrowhead) next to the nucleus above the Golgi. The Golgi cross-section appears normal. (H) Distended flagellar pocket.