Fig. 9. LUMA interacts with emerin. (A) Immunoprecipitation of different endogenous proteins from undifferentiated C2C12 cells with specific antibodies as indicated. Stars indicate antibody cross-reactive proteins, arrowhead points at IgGs covering presumable LUMA signal. Input of emerin results from a longer exposure. (B) Full-length LUMA 1-400, LUMA 53-309 or LUMAgal were overexpressed in HeLa cells and immunoprecipitated with antibodies against their C-terminal tags (V5 or Myc). Precipitates were analyzed for the presence of LUMA and emerin. LUMAe, endogenous LUMA; , LUMA degradation product (lanes 1 and 3, masked in lane 2 by LUMA 53-309); , IgG heavy chains; , LUMA 53-309 in lane 5 and C-terminal fragment of LUMAgal in lane 6. (C) Model for LUMA-emerin complexes at the INM.