Fig. 7. Xenopus NEDD1 (XNEDD1)-disruption inhibits microtubule organization in centrosome-independent aster assembly. (A) XNEDD1 and -tubulin immunofluorescence of centrosome-independent asters assembled in Xenopus egg extracts after addition of RanGTP. XNEDD1 localizes to the center of centrosome-independent asters and along their microtubules. Scale bar, 5 µm. (B) Microtubule structures assembled in XNEDD1-depleted extracts in response to RanGTP. Scale bar, 10 µm. (C) Microtubule structures assembled in response to RanGTP in extracts to which recombinant XNEDD1 (expressed and purified as a GST fusion protein; the GST portion was proteolytically removed prior to the experiment) had been added, as indicated. Scale bar, 10 µm.