Fig. 5. Transfection with a dominant-negative PKC mutant abrogates ezrin phosphorylation in T567 in CACO-2 C2BBe cells. The cells were transfected with a V5-tagged K274W PKC mutant 4 days after seeding and fixed 1 day later. The monolayers were processed with anti-T567-P ezrin antibody (green, A,C), and anti-V5 tag antibody (red, B,D), and counterstained with DAPI. The nuclei of V5-positive cells with various levels of V5 expression are labelled 1-4. Scale bar: 10 µm.