Fig. 1. L6-Ag regulates motility of breast cancer cells. (A) MCF-7 cells were co-transfected with plasmids encoding L6-Ag and GFP. Migration experiments towards fibronectin were performed 48 hours after transfection. (B,C) Cells were electroporated with control siRNA (si-Cont) or siRNA that targets L6-Ag (si-L6-Ag). Migration experiments were performed 72 hours after transfection. Migration was quantified by counting cells in seven random fields per membrane (10-30 cells/field) as described in detail in Materials and Methods. Data are reported as fold increases/decreases over migration of cells transfected with either control plasmid (GFP, A) or control siRNA (B,C). Data (all graph panels) are shown as mean ± standard deviation (s.d.) calculated from at least three separate experiments each performed in triplicate. P values were calculated using the two-tailed t-test. Right panels show representative western blots (WB) using lysates prepared 72 hours after transfection.