Fig. 5. Motility of L6-Ag-positive vesicles. MCF-7 cells were transiently transfected with the plasmid encoding GFP–L6-Ag and 48 hours later motility of the L6-Ag-positive endosomes was analysed by time-lapse video microscopy. Images were collected every 3 seconds for 1 minute (A) or every 3-5 seconds for 2 minutes (B). (A) Represents a superposition of the first and the last images artificially coloured in red and green, respectively. (B) Arrows point to a vesicle that fused with the enlarged L6-Ag-positive endosomes (video sequence). Scale bar: 10 µm.