Fig. 7. Effects of ORP3 overexpression in human primary macrophages. Human primary macrophages were transduced with AdGFP (A-C) or AdORP3 (D-F) recombinant adenoviruses. After 72 hours, the cells were fixed and stained for F-actin (Actin) and ORP3 (ORP3). The GFP expressed from both adenoviruses is shown in green (GFP). Scale bars: 20 µm. (G) Differentiated monocyte macrophages from three subjects were infected with AdORP3 or AdGFP for three days. Four million 3 µm polystyrene latex beads were loaded in each well containing 200,000 cells. After 1 hour, the cells were fixed, stained with AF568P, photographed and the beads were counted. The data represent the number of beads per cell, mean (± s.e.m.); the number of cells analyzed: Control (n=2169), AdORP3 (n=385), AdGFP (n=885). The P values indicate statistical significance.