Fig. 5. Reversal of Stim1 localization by ML-9 is complete. Shown are confocal images of cells in the presence of 1.8 mM extracellular Ca2+ (left panel), 15 minutes following store depletion with thapsigargin (Tg; 2 µM) in nominally Ca2+-free extracellular solution (center panel) and 5 minutes following addition of 100 µM ML-9 in the continued presence of Tg and Ca2+-free extracellular solution. Bars, 10 µm.