Fig. 9. Surface plasmon resonance (SPR) analysis of the binding of GST-PrP^23-107 to human LRP1. (A) Binding of 350 nM GST-PrP^23-107 compared with GST; dissociation started at 600 seconds. (B) Binding of 1 µM RAP, with a second addition of 1 µM RAP without (solid upper line) or with 100 nM GST-PrP^23-107 (dashed line); binding of 100 nM GST-PrP^23-107 alone is shown in the lower solid trace; dissociation started at 1150 seconds.