Fig. 3. TSP1 domain constructs. (A) Schematic diagrams of the TSP1 domain proteins. (B,C) Proteins were collected from conditioned medium of COS-7 cells on heparin-Sepharose (B) or Talon metal affinity resin (C) and analysed by SDS-PAGE and immunoblotting under reducing (R) or non-reducing (NR) conditions. Molecular mass markers are in kDa.