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Figure 4


Fig. 4. Knockdown of PITP{alpha} by siRNA decreases axonal growth in hippocampal neurons. E18 hippocampal neurons were nucleofected with indicated constructs or siRNAs, and cultured for 48 hours. (A) PITP{alpha} siRNA, but not control scrambled siRNA, leads to a reduction in endogenous PITP{alpha} levels, as validated by western blotting. (B) Knockdown of PITP{alpha} by siRNA significantly decreased axonal length (*P<0.0001, **P<0.0002). All data are the means ± s.e.m. of at least three independent experiments (n>125). (C) Knockdown of PITP{alpha} was monitored in each experiment by immunocytochemistry using anti-PITP{alpha} antibody. Scale bar: 20 µm.