Fig. 3. Last rupture event analysis in force-distance profiles is similar to analysis of all rupture events. Differentiated myofibroblasts grown on tipless AFM cantilevers were connected with differentiated myofibroblasts grown in monolayer for 2 seconds with constant approach velocity (0.1 µm/second) and loading force (3 nN). (A) Results obtained by including only the last rupture events in force-distance profiles (supplementary material Fig. S2) are displayed as Gaussian curve fits of histograms, normalized to the total number of last rupture events (n>1000). Force distribution is comparable to the histogram obtained after analyzing all rupture jumps in force-distance profiles (n5000) (Fig. 1I).