Fig. 2. DNA methylation is important for paternal PHE1 expression. (A) Relative PHE1 mRNA levels in wild-type flowers before fertilization (0 DAP) and siliques after pollination with wild-type or met-mutant pollen harvested at different days after pollination (DAP). Because the maternal PHE1 allele is silent, the detected PHE1 expression represents mostly the activity of the paternal PHE1 allele. (B) Allelic expression analysis of PHE1 after pollination of C24 plants with wild-type or met1-mutant pollen. Analysis was performed at DAP1 to DAP4.