Fig. 3. The 3' region of PHE1 contains DNA-methylation marks. (A, left) Southern blot of DNA from wild-type and met1-mutant plants after digestion with the methylation-sensitive restriction enzyme ClaI. (Right) Overview of the PHE1 locus with large and small arrows corresponding to the PHE1 coding region and repeats, respectively. The expected fragment sizes and the region covered by the probe are indicated. The methylated ClaI site is indicated by a filled circle, the repeat regions by arrows. (B) Cytosine methylation profile of the PHE1 downstream region in different tissues analyzed by bisulfite sequencing. Cytosine positions relative to the translational stop codon and sequence contexts are indicated on the x-axis. The ClaI site corresponds to position 2538. The methylation status in the met1 mutant was analyzed in flowers.