Fig. 4. Establishment of imprinting at the PHE1 locus is compromised in mutants whose PHE1 downstream region is disrupted. (A) Schematic overview of the location of the phe1-3-, phe1-4- and phe1-5-mutant alleles. Probes used for northern blot analysis are indicated. (B) Allelic expression analysis of PHE1 after reciprocal crosses of wild-type and phe1-3 mutant plants with the C24 accession. (C) Expression analysis of PHE1 in leaves of wild-type and phe1-3-mutant plants. (D) Northern blot analysis of RNA from leaves of wild-type and phe1-3-mutant plants with probes indicated in panel (A). (E) Allelic expression analysis of PHE1 in wild-type and phe1-4- and phe1-4-mutant plants after crosses of wild-type and mutant plants with the C24 accession. Lb, left border; Rb, right border; g, genomic DNA; ACT, ACTIN; wt, wild-type.