Fig. 3. Generation of mesenchymal lineages from cultured fetal liver cells. P2 passage cells cultured under conditions to induce adipogenic and osteogenic differentiation. Cells cultured under standard conditions did not show staining for Oil Red O (a) or Alizarin (c). Under differentiation induction conditions, cells began to accumulate lipid droplets and acquired a foamy appearance (b), which is typically seen in adipocytes, or exhibited calcium deposition (d), which is typically seen in osteocytes. Magnification: x200.