Fig. 5. Analysis of clonal EpCAM-positive cells. (a-c) Freshly isolated cells under phase (a), after GGT staining, red color, with hematoxylin counterstaining, blue color (b) and after glycogen staining (c). (d) An expanded colony of clonal P1 cells after culture for 6 weeks. (e-i) Cytospun preparations of cells released from late P1 culture with GGT (e) glycogen (f), as well as vimentin (g-i) staining in cells. The data verified that clonal cultured cells maintained epithelial characteristics while acquiring additional mesenchymal properties. Magnification: x200.