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Figure 2


Fig. 2. Growth arrest of MRC5-TERT cells under hyperoxia is telomere dependent. (A,B) ImmunoFISH of MRC5 cells (A) and MRC5-TERT cells (B) under hyperoxia (49 days and 42 days, respectively) showing telomeres (red) and {gamma}-H2A.X-containing foci (green). Boxed areas are shown at higher magnification on the right. Significant colocalisation of telomeres and {gamma}-H2A.X foci is indicated in white (arrowheads). (C) Average foci-telomere Pearson correlation coefficients for MRC5 fibroblasts in senescence under normoxia (MRC5 SEN), MRC5 cells after 7 weeks hyperoxia (MRC5 HYP), MRC5-TERT cells after 6 weeks hyperoxia (TERT HYP) and MRC5 cells 48 hours after 20Gy {gamma}-irradiation (MRC5 IR) as negative control. All experimental conditions are significantly different from the negative control, but not from each other. Data are mean±s.e.m., with the number of cells evaluated given for each condition.