Fig. 2. MEGAP mutant morphology and chemotaxis. (A) Vegetative morphology of mutant and wild-type (AX3) cell lines during axenic growth. Axenically growing cells were observed using Nomarski differential interference contrast (DIC). (B) Developed morphology of cell lines during under-agar chemotaxis. Pulse developed cells were observed during under-agar chemotaxis to 100 nM cAMP. (C) Chemotaxis of respective cell lines to a point source of 1 µM cAMP. cAMP-pulsed AX3 and mgp^– cells were resuspended in KK₂ medium and allowed to adhere to glass-bottomed dishes. Cells were then filmed for 10 minutes by DIC time-lapse microscopy, responding to a point source of cAMP delivered by micropipette. Images are representative of three experiments. Scale bars: 10 µm.