Fig. 5. Mutant MEGAP cells have more contractile vacuoles. (A) Contractile vacuole network of cells visualised with the lipophilic styryl dye FM2-10. Both mgp1^– and mgp1^–/2^– show many more contractile vacuoles than the wild type. Pictures are representative of at least three experiments. Scale bar: 10 µm. (B) Distribution of vacuole number in wild-type AX3 and mgp^– cells visualised with FM2-10. Average number of vacuoles per cell ± s.e.m. is shown in inset. The number of vacuoles in each cell was counted for 30 minutes. The distribution in B reflects the frequency of cells containing (n) vacuoles for all cells over this time. mgp1^– and mgp1^–/2^– have, on average, significantly more contractile vacuoles than the wild type (P=0.0012 and P=0.0052, respectively). GFP-MEGAP1 expressed in the mgp1^– background significantly rescues the contractile vacuole phenotype (P=0.0042).