Fig. 1. Lysis phenotypes of Agrho1a. (A) Genomic organization of RHO1 loci and neighboring genes in S. cerevisiae and A. gossypii. (B) Percent identity of Rho1 proteins derived from comparisons with the needle program of the EMBOSS package (Rice et al., 2000). (C) Representative mycelia of wild type, Agrho1a and Agrho1b. For the deletion mutants, spores from a heterokaryotic mycelium were spotted on medium selective for the marker of the deletion. (D) Hyphal lysis of Agrho1a growing on an AFM agar surface. Still images of individual hyphae that lysed during a time-lapse movie (Movie 1). Images were taken every 5 minutes. Sections of frames from time points just prior to and after lysis are shown. Bar, 25 µm. (E) Quantification of mycelial lysis. The alkaline phosphatase release of wild-type cells was set to 0% and the release of an Agslt2 to 100%. Values are given for at least three individual experiments.