Fig. 2. Actin-related phenotypes of Agrho1a. (A) A. gossypii hyphae grown for 20 hours in AFM and stained for filamentous actin with Alexa-Phalloidin. Bar, 10 µm. (B) Growth defect of an Agrho1a strain carrying AgABP140 fused to GFP. The control shows growth behavior without the plasmid. Cells were grown for at least 3 days on solid medium at 30°C. (C) Latrunculin A sensitivity of Agrho1a. A dense spore suspension of wild-type (upper panel) or Agrho1a spores (lower panel) was mixed with AFM-Agar and overlaid on AFM plates. Filter discs were placed on the petri dishes and the indicated amount of Latrunculin A was spotted on discs in a volume of 5 µl DMSO. Plates were then incubated at 25°C for about 36 hours. (D) Quantification of Latrunculin A sensitivity. The diameter of the inhibition zone was measured and plotted against the Latrunculin A concentration.