Fig. 1. (A-D) GFP-Kif5c localisation in living cells. Inverted fluorescence images are shown for COS-7 cells (A,B,D) and a neuronal cell (C) expressing GFP-Kif5c (supplementary material Movies 1-4). GFP-Kif5c shows a diffuse localisation but is excluded from organelles that show up as white in these inverted fluorescent images (black arrowheads in A and B). Some GFP-Kif5c is seen as puncta (grey arrows in B and C) along faintly labelled MTs, and in peripheral accumulations (black arrows in A and D). Curved MTs are also faintly labelled by GFP-Kif5c (to the left of the asterisk in B).