Fig. 1. PGE₂ stimulation mediated by EP2 and EP4 receptors or by cAMP elevation leads to podosome dissolution in DCs. (A-D) PGE₂ stimulation of iDCs leads to podosome loss and the appearance of FAs. (A,B) iDCs seeded on FN-coated coverslips were left untreated or were stimulated with PGE₂ for 5 minutes (min) or 16 hours (h) and stained with an anti-vinculin mAb (green) and phalloidin-Texas Red (to detect F-actin, red). (A) Representative images are depicted. (B) The number of cells displaying podosomes or FAs was counted in seven images per condition per experiment and an average (with s.e.m.) of six experiments is shown. (C) iDCs seeded on FN-coated coverslips were left untreated or were stimulated with PGE₂ for 5 minutes and stained with an anti-zyxin antibody (green) and phalloidin-Texas Red (to detect F-actin, red). (D) iDCs seeded on FN-coated coverslips were left untreated or were stimulated with PGE₂ for 1-5 minutes and stained with an anti-vinculin mAb and phalloidin-Texas Red (to detect F-actin). The number of cells displaying podosomes was determined in seven images per condition per experiment and an average (with s.e.m.) of four experiments is shown. Asterisks indicate significant differences (P<0.05).