Fig. 4. Expression constructs of MECP2 and their cellular localization. (A) Schematic showing the expression constructs of MECP2 that were used in the domain-deletion studies. Deleted residues based on murine MECP2e2 are shown in parentheses. The locations of NLS are shown as red boxes. (B) Cells expressing EGFP-tagged WT and mutant proteins were stained with DAPI and imaged by epifluorescence microscopy. The cellular distribution of the proteins is indicated (N, nuclear; HC, heterochromatin; C, cytoplasmic). (C) Deletion of the MBD results in the mislocalization of MECP2 to the nucleolus. Stable cell lines expressing EGFP-tagged WT and MBD were immunostained with the nucleolar marker nucleolin and counterstained with DAPI. (D) Cells expressing the TRD mutant showing both cytoplasmic and nuclear localization. Bars, 5 µm.