Fig. 5. Deletion of the N- or C-terminus of MECP2 does not have any significant impact on its mobility. (A) Cells expressing EGFP-tagged MECP2e2 (WT),
N and
C were photobleached and their recovery curves were plotted, revealing essentially no differences in binding kinetics for the deleted proteins. (B) Curve fitting was used to determine t50 values±s.d. for each of the protein forms (21.3±5.4, 17.6±9.3 and 23.9±7.8 for WT,
N and
C, respectively). The t50 values of
N and
C are not significantly different from WT (P>0.05). The number of nuclei photobleached in each group is shown within the bars.