Fig. 6. Deletion of the MBD, interdomain region and the TRD impact mobility of MECP2. (A) Recovery kinetics of the domain-deleted mutant forms indicate that that the MBD, ID and TRD contribute to the mobility. (B) The t⁵⁰ values for the domain-deleted proteins indicate increased mobility for the protein within heterochromatin domains for the ID and TRD proteins compared with WT. The t⁵⁰ for the MBD in nucleoplasm was intermediate between the value for euchromatin and heterochromatin for the WT protein (WT=21.3±5.4, MBD=1.3±0.4, ID=5.3±2.1 and TRD=7.9±2.5). Asterisks represent t⁵⁰ values that are significantly different from heterochromatin in WT (P<0.0001). Number of nuclei for each group is shown within each bar.