Fig. 7. Common RTT mutations significantly impact mobility of MECP2. (A) Recovery profiles of MECP2e2 (WT) and common RTT mutations of MECP2 found in the patient population. Balbc/3T3 cells were transiently transfected with CMV driven expression constructs encoding N-terminus EGFP-tagged MECP2, including common RTT mutations. Photobleaching was performed in regions of nucleoplasm (R106W, F155S, T158M) or heterochromatin (R133C, R168X). (B) The t⁵⁰ values of the WT and RTT mutants (WT=23.4±8.8, R106W=1.4±0.42, R133C=12.4±4.5, F155S=1.5±0.5, T158M=1.4±0.3 and R168X=1.17±0.5). The sample size for each group is given within the bars. Asterisks represent significant different values compared with the WT protein in heterochromatin (P<0.0001).