Fig. 1. GAS5 overexpression inhibits the growth of T-cell lines and promotes spontaneous apoptosis. (A) CEM-C7 cells were transiently transfected with GAS5 (Image clone 3585621) in pCMVSPORT6, or pCMVSPORT6 (without insert). Numbers of viable cells were determined by vital-dye staining (mean ± s.e.m. from five independent experiments). (B,C) Apoptosis was determined 48 hours post transfection using CaspaTag (mean ± s.e.m. from five independent experiments) of which representative photographs are shown in C. (D) DNA contents of CEM-C7/pCMVSPORT6 and CEM-C7/GAS5 were quantified by PI staining and fluorescence flow cytometry (mean ± s.e.m. from five independent experiments). Representative results are shown. (E) Colony-forming assay was performed 24 hours after transfection. Results are expressed as the mean ± s.e.m. and are representative of data obtained from five independent experiments. ^*P<0.01 compared with vector only.