Fig. 3. Overexpression of GAS5 induces growth arrest in Jurkat T-leukemic cells. (A,B) Jurkat T cells were transiently transfected with GAS5-01 constructs or with the vector only and numbers of colonies (A) and of viable cells (B) were determined. Data represent the mean ± s.e.m. from five independent experiments. (A,C) Long-term survival was assayed by a colony-forming assay (A) 24 hours after transfection, and DNA content from Jurkat/pCMVSPORT6- and Jurkat/GAS5-transfected cells was quantified by PI staining and fluorescence flow cytometry (C). Representative results from five experiments are shown. Data represent the mean ± s.e.m. from five independent experiments. ^*P<0.01 compared with vector only.