Fig. 5. The effect of hinge-region mutations on AR transactivation. COS-7 cells were transfected with GFP-tagged AR constructs, BOS β-galactosidase expression construct, and the androgen-responsive ARE₂-TATA-EIB-luc reporter gene. After treatment with 10 nM R1881 (black bars) or vehicle (grey bars) for 48 hours, cells were harvested and assayed for luciferase activity. Transfection efficiency was normalised to β-galactosidase activity and is shown relative to wtAR (^*). Mutations of the 630KLKK633 motif alter transactivation. P<0.001 compared with wtAR. P-values for all other samples were between 0.002 and 0.018.