Fig. 7. Reduced growth rates and premature death of tetop-LA^G608G bi-transgenic animals. Body weights (grams, A-C) or percent survival to age (weeks, D-F) were plotted for F1 line VF1-07. Each panel includes data from three different genotypes: (A) tetop-LA^G608G+; K5tTA⁺ (+/+); (B) tetop-LA^G608G+; K5tTA^– (+/–); and (C) tetop-LA^G608G–; K5tTA^– (–/–). The difference in survival for bi-transgenic animals is noted: (D) +/+ (n=12, median survival=14 weeks) and +/– (n=10), ²=12.79, P=0.0003. (E) +/+ (n=5, median survival=7 weeks) and +/– (n=9), ²=13.4, P=0.0003. (F) +/+ (n=6, median survival=29 weeks) and +/– (n=9), ²=7.943, P=0.0048. No statistically significant difference in survival was noted between +/– and –/– animals (D-F). Intercross breeding pairs were supplied with doxycycline that was removed at day of weaning – day 21 (A,D) or day of birth – day 0 (B,C,E,F). (C,F) Results when animals were fed on the cage floor with water-dissolved pellets from postnatal day 21 and onwards. (G) Photograph of 7-week-old bi-transgenic and single transgenic animals.