Fig. 12. Pak2 and Pak4 are phosphorylated downstream of PKC activation. (A,B) Extracts were prepared at 24 hours from ECs suspended in collagen matrices for 24 hrs in the absence and presence of TPA (A) or GF109203X (2.5 µM), Ro-32-0432 (5 µM), Go6983 (5 µM) or Go6976 (5 µM) (B) for western blot analysis and probed for phospho-Pak2, phospho-Pak4 or beta-actin (control).