Fig. 2. Colocalization of Anillin and RacGAP50C during cell division. (A) Cells were treated with DMSO (control) or Lat-A for 1 hour, and were then fixed and stained to detect Anillin (red in merged panels), tubulin (green in merged panels) and DNA (blue in merged panels). The rightmost panels show a magnification of the microtubules contacting the equatorial cortex in a Lat-A-treated cell; the spindle axis and chromosomes are not contained in this field. The arrowhead marks Anillin localization at the microtubule plus ends. (B) Cells were treated with DMSO (control) or Lat-A for 1 hour, and were then fixed and stained to detect Anillin (red in merged panels), RacGAP50C (green in merged panels) and DNA (blue in merged panels). The bracket and arrowheads mark the sites of Anillin and RacGAP50C colocalization (orange/yellow in merged panels). Note the colocalization at the microtubule plus ends in Lat-A-treated cells. (C) Cells were first incubated with dsRNA directed against RacGAP50C for 48 hours and then treated with DMSO (control) or Lat-A for 1 hour. The samples were then fixed and stained to detect Anillin (red in merged panels), tubulin (green in merged panels) and DNA (blue in merged panels). Note the absence of Anillin rod-shaped structure at the microtubule plus ends (marked by the arrow) in Lat-A-treated cells. Scale bars: 10 µm.