Fig. 9. The effect of specific kinase inhibitors on growth and focus formation of HDFs. (A) Focus formation of HDFs by E5 or v-Sis was assessed in the absence (–) or presence (+) of LY294002 (LY), wortmannin (W), SP600125 (SP) or Y27632. Foci were counted and averaged from two experiments. Focus formation is expressed as the mean percent (with standard error) relative to the number of foci formed by the untreated cells. (B-D) E5-expressing HDFs were seeded at an equal density either into 60 mM dishes (B) or a 24-well dish (C,D). Two days after plating, cells were either left untreated (–) or treated with the above inhibitors. At various times thereafter, cells were trypsinized and counted in triplicate. The mean cell number with standard error is plotted against the time after plating.