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Figure 4


Fig. 4. Morphology of the cysts and polarization of different marker proteins in knockdown cells. Columns 1. to 5. shown the following KD cells. 1., annexin-13 (Anx-13 KD); 2., caveolin-1 (Cav-1 KD); 3., galectin-3 (Gal-3 KD); 4., syntaxin-3 (Stx-3 KD); and 5., VIP17 KD. Panel groups A-D show immunostaining of the different KD cysts. Shown in green (AlexaFluor®488) are: A, apical membrane marker podocalyxin (Pcx); B, carcinoembryonic antigen (CEA); C, basolateral membrane marker E-cadherin (E-cad); D, tight-junction marker ZO-1. DAPI was used to stain nuclei (blue) and TRITC-phalloidin to show the actin meshwork (red). Arrowheads indicate (C) luminal and (D) aberrant localization of E-cadherin and ZO-1, respectively, observed sometimes in the different KDs (see text). Scale bars, 30 µm.