Fig. 3. The relationship between Nes expression levels and the 3D morphology of neural progenitor cells. (A) VZ cells were labeled with DiI (red) from the pial surface and observed for 4 hours after mitosis. The radial fiber, which became extremely thin during mitosis, was inherited by one of the daughter cells (arrowhead i). Four hours after mitosis, both daughter cells expressed dVenus. Within this period, the cell that inherited the process thickened its fiber and extended 20 µm (arrowhead i), whereas the cell that did not inherit the fiber began to elaborate its own process (arrowhead ii). (B) During G2 phase, the VZ progenitor cells possessed a mature fiber, and each nucleus migrated toward the ventricular surface. dVenus was not expressed in the cells during this phase (arrowhead). (C) Cells in M phase, labeled with phospho-histone H3 (PH3), were dVenus-negative both near the ventricular surface (arrowheads) and in the subventricular zone (arrow). The asterisk indicates the pial surface. Scale bar: 25 µm (A-C).