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Figure 2


Fig. 2. PECAM-1 isoforms lacking exon 15 are expressed in variable amounts in human and murine tissues. (A) Normal human tissue lysates were immunoblotted for {Delta}15 and total PECAM-1 protein. To determine {Delta}15 PECAM-1 expression in platelets, HUVECs and transformed human hematopoietic cells, antibody against PECAM-1 was incubated with whole cells, after which the cells were washed, lysed and cell-bound PECAM-1 was immunoprecipitated. PECAM-1 immunoprecipitates were probed for {Delta}15 and total PECAM-1 antigen. Note that varying relative amounts of {Delta}15 PECAM-1 are present in human ovary, brain and testes and that {Delta}15 PECAM-1 is on the surface of HUVECs, platelets and cancer cells. PECAM-1 antigen migrates with different electrophoretic mobilities in different tissues, probably owing to differences in glycosylation. (B) Murine PECAM-1 immunoprecipitates were probed for expression of total PECAM-1 and {Delta}15 PECAM-1. Note that a large proportion of the total PECAM-1 expressed in murine platelets and lung tissue is lacking exon 15. All results are representative of at least two independent experiments.