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Figure 3


Fig. 3. Protein interactions in a yeast two-hybrid assay. Saccharomyces cerevisiae strain AH109 were transformed with the indicated plasmid combinations containing the pGBKT7-binding domain (BD) and the pGADT7-activation domain (AD), and spotted onto SD minimal medium plates lacking either Leu and Trp or His, Leu and Trp supplemented with 0.75 mM 3-amino-1,2,4-triazole (3-AT) and grown at 30°C.