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Figure 7


Fig. 7. mRNA expression in Aedes ovaries. RT-PCR using RNA isolated from either previtellogenic (lanes b,e,h) or 16 hour post-blood-meal ovaries or with RNA derived from cultured Aag2 cells and primer pairs designed to amplify a 0.81 kb AaARH, 0.56 kb vitellogenin receptor (VgR) or a 0.5 kb AP-2 {alpha} subunit product. As controls, PCR with the identical primer sets and DNA plasmid template (lanes a,d,g) was performed. The position of the DNA standards (in kb) is indicated.