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Figure 5


Fig. 5. The localization of various Golgi proteins is altered in {Delta}ypt1/SLY1-20 cells. Cells were grown to early log phase at 23°C. One half of the culture was shifted to 37°C for 1 hour. Cells were either examined directly (Anp1p-GFP, Sed5p-GFP, Sec7p-dsRed) or treated for immunofluorescence (Emp47p-myc) with mouse anti-myc and goat-anti mouse IgGs coupled to CY3.