Fig. 2. Depletion of E-catenin has no effect on overall level of β-catenin or its nuclear localization. (A,A') Total protein lysates from E12.5 and E13.5 wild-type (WT) and E-catenin^–/– (KO) mouse brains were analyzed by western blotting with anti-E-catenin, anti-N-catenin, β-catenin and β-actin antibodies. Quantification of these results is shown in A'. Levels of β-catenin were normalized using β-actin and the results are shown as relative fold change. Data represent means ± s.d. (n=3). (B-C") Sagittal telencephalon sections from E13 embryos were immunostained for nuclear β-catenin. Sections were subjected to antigen retrieval, stained overnight with anti-β-catenin antibodies and processed using ABC MOM staining kit. Boxed areas in B and C are shown at higher magnification in B',B" and C',C", respectively. β-catenin was present in the nuclei of progenitor cells, which were localized around the ventricles. Prominent staining was also seen in the AJs at the ventricular surface (black arrows). There were no significant differences in the nuclear β-catenin between the wild-type (WT) and knockout (KO) mouse brains. HI, developing hippocampus. Scale bar in B: 0.12 mm for B,C and 0.012 mm for B'-C".