JCS -- Lien et al. 121 (9): 1357 Figure IG4

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Fig. 4. Transition from E12.5 to E13.5 results in extensive hyperplasia in ${alpha}$ E-catenin^–/– brains without significant changes in the expression of endogenous transcriptional targets of the β-catenin-signaling pathway. (A) Hyperplasia in E13.5 ${alpha}$ E-catenin^–/– mouse brains. Total cells were isolated from E12.5 and E13.5 heterozygous and ${alpha}$ E-catenin^–/– mouse brains and counted using a Coulter Counter. Data represent means ± s.d. n=3-5. ^*P<0.0001. (B) qPCR analysis of β-catenin pathway transcripts Myc, Ccnd1 (cyclin D1) and Axin2 in E12.5 and E13.5 heterozygous and ${alpha}$ E-catenin^–/– mouse brains. The levels of expression are shown in arbitrary units with mean of levels in heterozygous embryos adjusted to 1. Data represent means ± s.d. n=4.