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Figure 2


Fig. 2. Gβ{gamma} and G{alpha}q subunits dissociate upon carbachol stimulation in the presence of Cav1. (A) Pseudo-colored FRET images illustrating the time-dependence of energy transfer from eCFP-Gβ{gamma} to G{alpha}q-eYFP in acetylcholine (5 µM)-stimulated FRTcav+ (upper panel) and FRTcav– (lower panel) cells. The FRET color-scale is shown for comparison (red, 100%; black, 0%). For both series, the first panel was recorded before stimulation and the elapsed time between each image is 20 seconds. (B) Compiled results of the changes in NFRET from eCFP-Gβ{gamma} to G{alpha}q-eYFP in unstimulated and acetylcholine (5 µM)-stimulated FRTcav+ and FRTcav– cells with time. Studies were done using nine (FRTcav–) and four (FRTcav+) independent sets of samples (each measured in triplicate and averaged). (C) Pseudo-colored NFRET image illustrating energy transfer between free eCFP and eYFP.