Fig. 5. Immunofluorescence confocal images showing the colocalization of Cav1 and G_q. Confocal images of fixed FRT cells expressing transiently transfected Cav1-eGFP (green) are immunostained for endogenous G_q (red). Yellow pixels in the merged images indicate colocalization. Cells were either fixed without stimulation (top) or at 4 minutes after stimulation with 5 µm carbachol (bottom). Images were recorded with emission filter sets BP505-530 (green) and LP650 (red) using two separate lasers for sequential excitation (488 nm for green and 633 nm for red).