Fig. 5. β-catenin perturbs cell cycle progression in myogenic cells. The graphs show the quantification of the following experiments, and represent the mean ± s.e.m. of infected cells (satellite cells) or from at least ten random fields (plated satellite cells or C2 cells), all from three independent experiments. ^*P<0.05, significantly different from control cultures. Myofibre-associated satellite cells, plated satellite-cell-derived myoblasts or C2 cells were infected with pMSCV–β-catenin–IRES–eGFP, pMSCV–ST-β-catenin–IRES–eGFP or pMSCV-IRES-eGFP. In myofibre-associated satellite-cell cultures, BrdU was added after 72 hours of culture for 3 hours, and cells were then fixed and immunostained. BrdU incorporation was significantly reduced by constitutively expressed β-catenin expression. For plated satellite cells and C2 cells, infection was followed the next day by culture in mitogen-poor medium for at least another 4 days before the BrdU pulse. The presence of β-catenin further reduced the number of cells able to incorporate BrdU.