Fig. 6. When degradation of endogenous β-catenin is inhibited satellite-cell self-renewal is promoted. (a,b,d,e) Myofibres were cultured in plating medium containing the GSK3β inhibitor SB216763 for 3 days, then fixed and immunostained. Inhibition of β-catenin phosphorylation and, therefore, degradation, significantly increased the number of (a) Pax7⁺MyoD^– cells compared with (b) control cells cultured without drug. Stabilisation of β-catenin significantly reduced the number of myogenin-positive (Myog⁺) cells, whereas increasing (d) Pax7⁺–myogenin-negative (Pax7⁺Myog^–) cells compared with (e) control cells. Nuclei were counterstained with DAPI. (c,f) Quantification of experiments shown in a,b (c), and in d,e (f). Data are the mean ± s.e.m. from three independent experiments. ^*P<0.05, significantly different from control cultures.