Fig. 3. slk1 is required for spore formation. (A) The slk1 mutant produces asci with small spores. Wild-type (S1478) and slk1 (S1883) h⁹⁰ homothallic strains were incubated on sporulation media (MEA) for 2 days, and Nomarski microphotographs were taken. Scale bar: 10 µm. (B) The slk1 mutant proceeds through meiotic divisions with normal kinetics. Meiosis progression in pat1-114 diploid strains S964 (wild type) and S1566 (slk1) was monitored by DAPI staining. Percentages of cells with one nucleus (green square), two nuclei (red triangle; meiosis I) and three to four nuclei (blue circle: meiosis II) are shown.